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Anna Berry, MD, Medical Director of Molecular Diagnostics, CellNetix Pathology & Laboratories Danbin Xu, MD, PhD, Technical Director, Molecular Pathology, CellNetix Pathology & Laboratories

Next Generation Sequencing in Clinical Oncology


By Anna Berry, MD
Medical Director of Molecular Diagnostics, CellNetix Pathology & Laboratories

By Danbin Xu, MD, PhD
Technical Director, Molecular Pathology, CellNetix Pathology & Laboratories

Next generation sequencing (NGS), often referred to as massively parallel sequencing, is a relatively new technique in molecular diagnostics that has truly served as a “disruptive technology” in the area of cancer treatment. Prior to the availability of NGS, one small fragment of DNA could be sequenced per reaction, using a technique called Sanger sequencing, that at the time of its discovery was also quite revolutionary. In Sanger sequencing, terminator nucleotides are used randomly in a PCR (polymerase chain reaction) reaction to create fragments of differing lengths with a known base at the terminal end. The fragments can then be lined up to deduce the sequence of the entire region being targeted. In contrast, NGS can sequence millions of fragments simultaneously, by sequentially adding reversible terminator nucleotides to numerous fragments of DNA from different samples, throughout all regions of interest, in one reaction. This is accomplished by adding adapters to the ends of the DNA fragments (used to affix fragments to a fixed surface, to “barcode” individual samples within a larger reaction, and to prime the PCR reactions), and then adding the reversible terminator nucleotides one by one, measuring the addition, and then cleaving the “terminator” portion so that another nucleotide can be added.  Read article
Two executives in outside business meeting

Regression in Senior Leaders: Positional Power as a Last Resort

By Douglas Riddle, Ph.D.
Global Director, Coaching Services
Center for Creative Leadership

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Pen, financial column, calculator

For a Picture of Your Organization’s Financial Health, Check Your Balance Sheet

By Eric Nicholson, Moss Adams LLP
By Aparna Venkateswaran, Moss Adams LLP
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Young girl sleeping in hospital bed

Creating a Quieter Hospital: Enloe Medical Center 2009-2014

By Marcia Nelson MD MMM CPE FAAFP FACPE
Vice President of Medical Affairs
Enloe Medical Center

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